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Casein Isolation and Hydrolysis Experiment by Nhess Estrada is a document available to read on EtoBox.

What is Casein Isolation and Hydrolysis Experiment about?

The document summarizes an experiment to isolate casein from powdered milk, hydrolyze it, and neutralize it. Casein was first isolated by dissolving powdered milk in warm water and acidifying it with acetic acid until the pH reached 4.6, causing the casein to precipitate out. The isolated casein was then hydrolyzed using sulfuric acid and autoclaving. Finally, the hydrolyzed casein was neutralized by adding barium hydroxide to raise the pH to 7. The percentage yield of isolated casein was calculated to be 7

Author
Nhess Estrada
Language
EN