Can I read A Streamlined Approach to the Analysis of Volatile Fatty Acids and Its Application to the Measurement of Whole‐body Flux on EtoBox?
A Streamlined Approach to the Analysis of Volatile Fatty Acids and Its Application to the Measurement of Whole‐body Flux by Douglas J. Morrison; Karen Cooper; Susan Waldron; Christine Slater; Lawrence T. Weaver; Tom Preston is a Chemistry article available to read on EtoBox.
What is A Streamlined Approach to the Analysis of Volatile Fatty Acids and Its Application to the Measurement of Whole‐body Flux about?
## Abstract Volatile fatty acids (VFAs) are produced in the human colon by the bacterial breakdown of carbohydrates that escape digestion and absorption in the small intestine. They have important local and systemic effects on gastrointestinal and nutritional functions. Measuring their production is difficult because of inaccessibility of sampling sites and low circulating concentrations. Stable isotope tracer techniques are a way to measure VFA production but require measurement of isotope dilution in blood and other biological fluids. We have developed a streamlined and robust method to measure the concentration and enrichment of [^2^H]‐labelled VFAs by gas chromatography/mass spectrometry (GC/MS) and [^13^C]‐labelled VFAs by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). Both types of analysis were carried out on the same samples allowing multiple tracer studies to be conducted. Good accuracy and repeatability were found for GC/MS analysis of [^2^H]‐labelled VFAs. Careful handling of the background contribution, especially acetate, allowed quantitation of concentration and enrichment within the analysis. GC/C/IRMS analysis of [^13^C] VFAs was also ach
Who reads A Streamlined Approach to the Analysis of Volatile Fatty Acids and Its Application to the Measurement of Whole‐body Flux?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Douglas J. Morrison; Karen Cooper; Susan Waldron; Christine Slater; Lawrence T. Weaver; Tom Preston
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2004
- Language
- EN
- Field
- Chemistry (Physical Sciences)