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Can I read 130 Development of a minimally-invasive method, guided by in vivo non-invasive imaging, to sample atopic skin on EtoBox?
130 Development of a minimally-invasive method, guided by in vivo non-invasive imaging, to sample atopic skin by O. Yélamos; D. Andersen; P. Iglesias; M. Potrony; M. Dominguez; A. Herrero; B. Alejo; J. Mateu; M. Røpke; M. Pont Giralt; N. Banhos Danneskiold-Samsøe; K. Kristiansen; J. Malvehy; R. Guy; S. Brix; S. Puig is a Medicine article available to read on EtoBox.
What is 130 Development of a minimally-invasive method, guided by in vivo non-invasive imaging, to sample atopic skin about?
Abnormal autophagy is observed in several skin diseases associated with hyperproliferation. We have shown that Beclin1 is increased in psoriatic lesions and when Beclin1 is conditionally knocked out in the epidermis, imiquimod(IMQ)-induced hyperproliferation is markedly attenuated. This indicates that autophagy plays a positive role in psoriasis and contributes to epidermal hyperproliferation in psoriatic lesions. However, how autophagy is regulated in the epidermis of psoriatic lesions is a knowledge gap that needs to be filled. We show that loss of EphA2, a receptor tyrosine kinase, reduces proliferation in mouse epidermis and human keratinocytes. Importantly, expression of EphA2 was shown to be increased in psoriatic lesions. We demonstrate that knockdown of EphA2 in HEKs results in an increase in: (i) large vacuoles; and (ii) LC3II protein, a marker for autophagosomes. To further explore this defect in autophagy, we measured autophagy flux by two well-established approaches: LC3II turnover and p62 levels, and found that loss of EphA2 reduced LC3II turnover in HEKs; and increased p62 in HEKs as well as in mouse epidermis, indicating an inhibition of autophagy flux. To determine
Who reads 130 Development of a minimally-invasive method, guided by in vivo non-invasive imaging, to sample atopic skin?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- O. Yélamos; D. Andersen; P. Iglesias; M. Potrony; M. Dominguez; A. Herrero; B. Alejo; J. Mateu; M. Røpke; M. Pont Giralt; N. Banhos Danneskiold-Samsøe; K. Kristiansen; J. Malvehy; R. Guy; S. Brix; S. Puig
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)