About this Immunology and Microbiology article

Thymosin- α 1 stimulates maturation of CD34+ stem cells into CD3+4+ cells in an in vitro thymic epithelia organ coculture model by Alan P Knutsen; John J Freeman; Kathleen R Mueller; Stanford T Roodman; John D Bouhasin is a Immunology and Microbiology article available to read on EtoBox.

The e}ect of thymosin!a 0 on thymopoiesis is largely unknown[ Thymosin is found in the cortical and medullary thymic epithelia\ as well as in nurse cells^thus\ it is hypothesized that thymosin may a}ect both early and late stage of thymocyte maturation[ In this study\ the e}ect of thymosin!a 0 on thymopoiesis was determined by coculturing in vitro CD23 ¦ stem cells "SC# with allogeneic cultured thymic epithelia fragments "CTEF# for 0Ð3 weeks and analyzing T!cell maturation by ~ow cytometry[ Thymosin!a 0 signi\_cantly enhanced the cell number "e[g[\ proliferation# of mononuclear cells obtained at 1 and 3 weeks of the SC!CTEF cocultures "P 3 9[90 and 3 9[94\ respectively#[ In particular\ thymosin!a 0 stimulated expression of CD2 ¦ cells at 2 and 3 weeks "P 3 9[94#[ The predominant subpopulation increased by thymosin stimulation was single positive mature CD3 ¦ cells\ which was con\_rmed to occur within the SC!CTEF thymic organ tissue by laser confocal immuno~uorescence microscopy[ Thymosin stimulation tended to enhance IL!6 synthesis\ critical cytokine in the maturation of thymocytes[ In summary\ this is the \_rst study to demonstrate that thymosin!a 0 enhanced thymopoiesis of CD23 ¦

Author

Alan P Knutsen; John J Freeman; Kathleen R Mueller; Stanford T Roodman; John D Bouhasin

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0192-0561)

Published

1999

Language

EN

Field

Immunology and Microbiology (Life Sciences)