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15N-enrichment of plant tissue to mark phytophagous insects, associated parasitoids, and flower-visiting entomophaga by Shawn A. Steffan; Kent M. Daane; Daniel L. Mahr is a Agricultural and Biological Sciences article available to read on EtoBox.

What is 15N-enrichment of plant tissue to mark phytophagous insects, associated parasitoids, and flower-visiting entomophaga about?

## Abstract New techniques are presented on the use of ^15^N to mark insects. ^15^N, a stable isotope of nitrogen, was enriched above natural abundance in plant and insect tissues. Two laboratory studies demonstrated that enriched ^15^N‐concentrations could be tracked from plant to insect using mass spectrometry. In the first study, adult __Cotesia plutellae__ (Kurdjimov) (Hymenoptera: Braconidae) and __Hippodamia convergens__ Guérin‐Méneville (Coleoptera: Coccinellidae) were allowed to feed at the flowers of rapid‐cycling Chinese cabbage plants that had been fertilized with ^15^N‐enriched potassium nitrate (KNO~3~‐^15^NO~3~). Both insect groups were found to have significantly elevated ^15^N levels after visiting the flowers of the ^15^N‐enriched plants for 48 hours. In the second study, ^15^N‐enriched bean plant (__Phaseolus vulgaris__ L.) tissue was incorporated into an insect diet and fed to navel orangeworms, __Amyelois transitella__ (Walker) (Lepidoptera: Pyralidae). When the navel orangeworm larvae were 4th instars, they were removed from the diet and exposed to the parasitoid, __Goniozus legneri__ Gordh (Hymenoptera: Bethylidae). Results indicated that the enriched ^15^N‐co

Who reads 15N-enrichment of plant tissue to mark phytophagous insects, associated parasitoids, and flower-visiting entomophaga?

Author

Shawn A. Steffan; Kent M. Daane; Daniel L. Mahr

Publisher

Springer; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0013-8703)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)