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Molecular cloning of the calcium and sodium ATPases in Neurospora crassa by Begoña Benito; Blanca Garciadeblás; Alonso Rodríguez‐Navarro is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Molecular cloning of the calcium and sodium ATPases in Neurospora crassa about?

Using PCR, reverse transcription‐PCR (RT‐PCR) and colony hybridization in a genomic library, we isolated six genes which encode type II P‐type ATPases in __Neurospora crassa__. The six full‐length cDNAs were cloned in a yeast expression vector and transformed into __Saccharomyces cerevisiae__ null Ca^2+^‐ or Na^+^‐ATPase mutants. Three cDNAs suppressed the defect of the Ca^2+^ mutant and two of these protected from Mn^2+^ toxicity. One cDNA suppressed the defect of the Na^+^ mutant and two cDNAs were not functional in __S. cerevisiae__. The expression of the transcripts of the six genes in the presence of Ca^2+^, Na^+^, high pH or supporting an osmotic shock indicated that, with the exception of one of the Ca^2+^‐ATPases, the main function of the cloned ATPases is the adaptation to stress conditions. The relationship between the cloned fungal Ca^2+^‐ and Na^+^‐ATPases and plant type II P‐ATPases is discussed.

Who reads Molecular cloning of the calcium and sodium ATPases in Neurospora crassa?

Author

Begoña Benito; Blanca Garciadeblás; Alonso Rodríguez‐Navarro

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)