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Fusion of Formate Dehydrogenase and Alanine Dehydrogenase as an Amino Donor Regenerating System Coupled to Transaminases by Valentina Marchini; Ana I. Benítez‐Mateos; Sofia L. Hutter; Francesca Paradisi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Fusion of Formate Dehydrogenase and Alanine Dehydrogenase as an Amino Donor Regenerating System Coupled to Transaminases about?

## Abstract Fusion enzymes are attractive tools for facilitating the assembly of biocatalytic cascades for chemical synthesis. This approach can offer great advantages for cooperative redox cascades that need the constant supply of a donor molecule. In this work, we have developed a self‐sufficient bifunctional enzyme that can be coupled to transaminase‐catalyzed reactions for the efficient recycling of the amino donor (L‐alanine). By genetic fusion of an alanine dehydrogenase (AlaDH) and a formate dehydrogenase (FDH), a redox‐complementary system was applied to recycle the amino donor and the cofactor (NADH), respectively. AlaDH and FDH were assembled in both combinations (FDH‐AlaDH and AlaDH‐FDH), with a 2.5‐fold higher enzymatic activity of the latter system. Then, AlaDH‐FDH was coupled to two different __S__‐selective transaminases for the synthesis of vanillyl amine (10 mM) reaching up to 99 % conversion in 24 h in both cases. Finally, the multienzyme system was reused for at least 3 consecutive cycles when implemented in dialysis‐assisted biotransformations.

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Author

Valentina Marchini; Ana I. Benítez‐Mateos; Sofia L. Hutter; Francesca Paradisi

Publisher

Wiley

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)