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Developmental heat stress interrupts spermatogenesis inducing early male sterility in Drosophila melanogaster 1 1 Introduction 1 2 Materials and methods 2 2.1 Fly stocks and culturing 2 2.2 General procedures to generate experimental males 2 2.3 Male reproductive tract measures during early adult recovery 2 2.3.1 Sperm presence and location in the testes 2 2.3.2 Sperm area in the SV 2 2.4 Recovery potential of different developmental stages after heat stress 3 2.4.1 Male reproductive tract measures during recovery – SV size and sperm presence in the SV after development at 30 °C 4 2.5 Data analysis 4 3 Results 4 3.1 Male reproductive tract measures during early adult recovery 4 3.1.1 Sperm presence and location in the testes 4 3.1.2 Sperm area in the SV 4 3.2 Recovery potential of different developmental stages after heat stress 5 3.2.1 Male reproductive tract measures during recovery – SV size and sperm presence in the SV after development at 30 °C 5 4 Discussion 6 5 Conclusions 8 Data accessibility statement 8 Funding statement 8 CRediT authorship contribution statement 8 Declaration of competing interest 8 Acknowledgements and funding statement 8 Appendix A Supplementary data

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UND

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nonfiction