Can I read Genetic and Physical Mapping of the Patatin Genes in Potato and Tomato on EtoBox?

Genetic and Physical Mapping of the Patatin Genes in Potato and Tomato by Martin W. Ganal; Meredith W. Bonierbale; Marion S. Roeder; William D. Park; Steven D. Tanksley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Genetic and Physical Mapping of the Patatin Genes in Potato and Tomato about?

Genes for the major storage protein of potato, patatin, have been mapped genetically and physically in both the potato and tomato genomes. In potato, all patatin genes detected by the cDNA clone pGM01 map to a single locus at the end of the long arm of chromosome 8. By means of pulsed field gel electrophoresis (PFGE) it was possible further to delimit this locus, containing 10-15 copies of the gene, to a maximum size of 1.4 million base pairs. Hybridizations with class-specific clones suggest that the locus is at least partially divided into domains containing the two major types of patatin genes, class I and II. In tomato, patatin-homologous sequences were found to reside at the orthologous locus at the end of chromosome 8. The approximately three copies in tomato were localized by PFGE to a single fragment of 300 kilobases. Whereas the class II-specific 5' promoter sequences reside in tomato at the same locus as the coding sequences, the single class I-specific copy of the 5' promoter sequences was localized on chromosome 3 with no coding sequence attached to it. A clone from this chromosome 3 locus of tomato was isolated and by restriction fragment length polymorphism mapping it

Who reads Genetic and Physical Mapping of the Patatin Genes in Potato and Tomato?

Author

Martin W. Ganal; Meredith W. Bonierbale; Marion S. Roeder; William D. Park; Steven D. Tanksley

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)