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Gas Chromatographic Separation of Fatty Acid Methyl Esters on Weakly Polar Capillary Columns by Kouhei Yamamoto; Akemi Kinoshita; Akira Shibahara is a Chemistry article available to read on EtoBox.

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It was found that weakly polar columns, routinely used in capillary GC for analyzing sterols, food additives, etc., can also be used for separating fatty acid methyl esters (FAMEs). On these columns, FAMEs elute in the order of their unsaturation. The equivalent chain-length value of methyl 22:6 is below 23.00. This means FAMEs within a carbon chain length, having up to six double bonds, elute before the next (one carbon longer) saturated FAME elutes. Peak identification is easy. Weakly polar columns are compatible in both GC and GC/MS systems.

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Author

Kouhei Yamamoto; Akemi Kinoshita; Akira Shibahara

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)