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Exosomal‐miR‐522‐3p derived from cancer‐associated fibroblasts accelerates tumor metastasis and angiogenesis via repression bone morphogenetic protein 5 in colorectal cancer by Jun Zhang; Yuliang Pan; Long Jin; Huiyun Yang; Peiguo Cao is a Medicine article available to read on EtoBox.

What is Exosomal‐miR‐522‐3p derived from cancer‐associated fibroblasts accelerates tumor metastasis and angiogenesis via repression bone morphogenetic protein 5 in colorectal cancer about?

## Abstract ## Background Colorectal cancer (CRC) is a gastrointestinal tract malignancy. Exosomes secreted by cancer‐associated fibroblasts (CAFs) are reported to participate in tumor progression by delivering noncoding RNA or small proteins. However, the function of exosomal miR‐522‐3p in CRC remains unclear. ## Methods CAFs were derived from tumor tissues, and exosomes were identified by western blot or TEM/NTA and originated from CAFs/NFs. The viability, invasion, and migration of HUVECs and CRC cells was examined using MTT, Transwell, and wound healing assays, respectively. The molecular interactions were validated using dual luciferase reporter assay and RIP. Xenograft and lung metastasis mouse models were generated to assess tumor growth and metastasis. ## Results Exosomes extracted from CAFs/NFs showed high expression of CD63, CD81, and TSG101. CAF‐derived exosomes significantly increased the viability, angiogenesis, invasion, and migration of HUVECs and CRC cells, thereby aggravating tumor growth, invasion, and angiogenesis in vivo. miR‐522‐3p was upregulated in CAF‐derived exosomes and CRC tissues. Depletion of miR‐522‐3p reversed the effect of exosomes derived from CAFs

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Author

Jun Zhang; Yuliang Pan; Long Jin; Huiyun Yang; Peiguo Cao

Publisher

Wiley

Published

2023

Field

Medicine (Health Sciences)