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Tip60 Promotes Prostate Cancer Cell Proliferation by Translocation of Androgen Receptor Into the Nucleus by Masaki Shiota; Akira Yokomizo; Daisuke Masubuchi; Yasuhiro Tada; Junichi Inokuchi; Masatoshi Eto; Takeshi Uchiumi; Naohiro Fujimoto; Seiji Naito is a Medicine article available to read on EtoBox.

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## Abstract ## BACKGROUND There are currently few effective therapies for castration‐resistant prostate cancer (CRPCa). CRPC which is resistant to castration is thought to result from increased activation of the androgen/androgen receptor (AR) signaling pathway, which may be augmented by AR coactivators. ## METHODS Luciferase reporter assay, Western blotting, quantitative real‐time polymerase chain reaction, fluorescence microscopy, cell proliferation assay, and flow cytometry for cell‐cycle analysis were used to resolve a role of Tip60 regulating AR in PCa cells. ## RESULTS Tip60 regulated transcriptions of AR target genes androgen independently. Tip60 knockdown induced translocation of AR into the cytoplasm. Acetylation‐mimicking mutations in the nuclear localization signal sequence caused AR protein to mainly localize in the nucleus despite androgen starvation, whereas non‐acetylation‐mimicking mutations caused AR to mainly localize in the cytoplasm despite androgen stimulation. Tip60 overexpression in castration‐resistant LNCaP derivative CxR cells resulted in increases in the acetylated form of AR and AR localization in the nucleus even without androgen. Consequently, Tip60 si

Who reads Tip60 Promotes Prostate Cancer Cell Proliferation by Translocation of Androgen Receptor Into the Nucleus?

Author

Masaki Shiota; Akira Yokomizo; Daisuke Masubuchi; Yasuhiro Tada; Junichi Inokuchi; Masatoshi Eto; Takeshi Uchiumi; Naohiro Fujimoto; Seiji Naito

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2009

Language

EN

Field

Medicine (Health Sciences)