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Analysis of the Effect of Potato Carboxypeptidase Inhibitor Pro-sequence on the Folding of the Mature Protein by Sílvia Bronsoms; Josep Villanueva; Francesc Canals; Enrique Querol; Francesc X. Aviles is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Analysis of the Effect of Potato Carboxypeptidase Inhibitor Pro-sequence on the Folding of the Mature Protein about?

Protein folding can be modulated __in vivo__ by many factors. While chaperones act as folding catalysts and show broad substrate specificity, some pro‐peptides specifically facilitate the folding of the mature protein to which they are bound. Potato carboxypeptidase inhibitor (PCI), a 39‐residue protein carboxypeptidase inhibitor, is synthesized __in vivo__ as a precursor protein that includes a 27‐residue N‐terminal and a seven‐residue C‐terminal pro‐regions. In this work the disulfide‐coupled folding of mature PCI __in vitro__ has been compared with that of the same protein extended with either the N‐terminal pro‐sequence (ProNtPCI) or both N‐ and C‐terminal pro‐sequences (ProPCI), and also with the N‐terminal pro‐sequence in __trans__ (ProNt + PCI). No significant differences can be observed in the folding kinetics or efficiencies of all these molecules. In addition, __in vivo__ folding studies in __Escherichia coli__ have been performed using wild‐type PCI and three PCI mutant forms with and without the N‐terminal pro‐sequence, the mutations had been previously reported to affect folding of the PCI mature form. The extent to which the ‘native‐like’ form was secreted to the medi

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Author

Sílvia Bronsoms; Josep Villanueva; Francesc Canals; Enrique Querol; Francesc X. Aviles

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)