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Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) -jeffamine® hydrogel beads by Guohua Chen; Leen Van Der Does; Adriaan Bantjes is a Materials Science article available to read on EtoBox.

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Hydrogel-like polymers with reactive cyclic carbonate ( CCA) groups have been obtained by the cross-linking reaction of poly(viny1ene carbonate) (PVCA) with Jeffamines@. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.

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Author

Guohua Chen; Leen Van Der Does; Adriaan Bantjes

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-8995)

Published

1993

Language

EN

Field

Materials Science (Physical Sciences)