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Tetramer formation of Bacillus subtilis YabJ protein that belongs to YjgF/YER057c/UK114 family by Zui Fujimoto; Le Thi Thu Hong; Naomi Kishine; Nobuhiro Suzuki; Keitarou Kimura is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Tetramer formation of Bacillus subtilis YabJ protein that belongs to YjgF/YER057c/UK114 family about?

## ABSTRACT Bacillus subtilis YabJ protein belongs to the highly conserved YjgF/YER057c/UK114 family, which has a homotrimeric quaternary structure. The dominant allele of yabJ gene that is caused by a single amino acid mutation of Ser103Phe enables poly-γ-glutamic acid (γPGA) production of B. subtilis under conditions where the cell-density signal transduction was disturbed by the loss of DegQ function. X-ray crystallography of recombinant proteins revealed that unlike the homotrimeric wild-type YabJ, the mutant YabJ(Ser103Phe) had a homotetrameric quaternary structure, and the structural change appeared to be triggered by an inversion of the fifth β-strand. The YabJ homotetramer has a hole that is highly accessible, penetrating through the tetramer, and 2 surface concaves as potential ligand-binding sites. Western blot analyses revealed that the conformational change was also induced in vivo by the Ser103Phe mutation.

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Author

Zui Fujimoto; Le Thi Thu Hong; Naomi Kishine; Nobuhiro Suzuki; Keitarou Kimura

Publisher

Informa UK Limited

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)