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Effects of hydrolyzed fish protein and autolyzed yeast as substitutes of fishmeal in the gilthead sea bream ( Sparus aurata ) diet, on fish intestinal microbiome by Rimoldi, S.; Gini, E.; Koch, J. F. A.; Iannini, F.; Brambilla, F.; Terova, G. is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Background This study evaluated the effects of partial substitution of dietary fishmeal (FM) with either fish protein hydrolysate (FPH) or autolysed dried yeast (HiCell®, Biorigin, Brazil) on intestinal microbiota of gilthead sea bream (__Sparus aurata__). A total number of 720 fish of 122.18 ± 6.22 g were fed for 92 days with three different diets in triplicate (3 tanks/diet). A diet based on FM/vegetable meal was used as control. The other two diets were formulated by replacing FM with 5% of either FPH or HiCell®. To analyze the gut microbiota associated to autochthonous and allochthonous microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME pipeline were used. ## Results A total number of 102 OTUs (operational taxonomic units) at 97% identity were identified in fish gut samples collected at the end of feeding trial. Fourteen OTUs constituted the core gut microbiota, i.e. those OTUs found in at least nine out of fifteen samples per group and shared regardless of the diet. Eight OTUs were assigned to __Firmicutes__ represented by __Lactobacillus__, __Staphylococcus__, and __Bacillus__ genera, and six to __Proteobacteria__ phylum. Dietary d

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Author

Rimoldi, S.; Gini, E.; Koch, J. F. A.; Iannini, F.; Brambilla, F.; Terova, G.

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2005-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1746-6148)

Published

2020

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)