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Transcriptional element H4-site II of cell cycle regulated human H4 histone genes is a multipartite protein/DNA interaction site for factors HiNF-D, HiNF-M, and HiNF-P: Involvement of phosphorylation by Andre J. van Wijnen; Anna L. Ramsey-Ewing; Rita Bortell; Thomas A. Owen; Jane B. Lian; Janet L. Stein; Gary S. Stein is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Cell cycle regulated gene expression was studied by analyzing protein/DNA interactions occurring at the H4‐Site II transcriptional element of H4 histone genes using several approaches. We show that this key proximal promoter element interacts with at least three distinct sequence‐specific DNA binding activities, designated HiNF‐D, HiNF‐M, and HiNF‐P. HiNF‐D binds to an extended series of nucleotides, whereas HiNF‐M and HiNF‐P recognize sequences internal to the HiNF‐D binding domain. Gel retardation assays show that HiNF‐D and HiNF‐M each are represented by two distinct protein/DNA complexes involving the same DNA binding activity. These results suggest that these factors are subject to post‐translational modifications. Dephosphorylation experiments in vitro suggest that both electrophoretic mobility and DNA binding activities of HiNF‐D and HiNF‐M are sensitive to phosphatase activity. We deduce that these factors may require a basal level of phosphorylation for sequence specific binding to H4‐Site II and may represent phosphoproteins occurring in putative hyper‐ and hypo‐phosphorylated forms. Based on dramatic fluctuations in the ratio of the two distinct HiNF‐D specie

Who reads Transcriptional element H4-site II of cell cycle regulated human H4 histone genes is a multipartite protein/DNA interaction site for factors HiNF-D, HiNF-M, and HiNF-P: Involvement of phosphorylation?

Author

Andre J. van Wijnen; Anna L. Ramsey-Ewing; Rita Bortell; Thomas A. Owen; Jane B. Lian; Janet L. Stein; Gary S. Stein

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)