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Conditions Affecting the Extraction and Molecular Weight of Ribonucleic Acid from Mammalian Tissues by Jean Lust is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## JEAN LUST alone. These experiments establish precise conditions for use of SDS and handling of tissue required to obtain a sizable amount of insoluble RNA. Characterization of this RNA strongly suggests that it contains nuclear RNA as well as cytoplasmic RNA. ## Methods Preparation and Disruption of Frozen Powder of Rat Liver by SDS. SDS was prepared by purification of Duponol C" according to the procedure described by Crestfield, Smith, and Allen (1) and dissolved in 0.0125 M Na,HPO,-0.0125 M NaH,PO, in concentrations varying between 0.1 and 2 gm% (w/v). Rats from the Long Evans, Nelson ( 27) or Fisher Agouti strains were fasted for 20-24 hr. The livers were removed under ether anesthesia and frozen in a Dewar flask containing dry ice and alcohol. A frozen powder of 0.5 gm of liver Gssue was prepared by crushing frozen liver with a mortar and pestle in a dry-ice chest. A test tube with the powder was introduced quickly into a boiling water bath. Immediately thereafter, 4 ml of SDS, which had reached the temperature of the water bath, were poured on the powder. Heating time with vigorous stirring in the water bath lasted between 3 and 10 min. The stirring device consisted of a n

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Author

Jean Lust

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1965

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)