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Biofilm inhibition in Candida albicans with biogenic hierarchical zinc-oxide nanoparticles 1 1. Introduction 1 2. Experimental sections 2 2.1. Chemicals used in the study 2 2.2. Microwave-assisted extraction of lignin from bagasse 2 2.3. Fragmentation of lignin 2 2.4. Oxidation of fragmented lignin (FL) 2 2.5. Synthesis of zinc oxide nanoparticles (ZnO NPs) 2 2.6. Characterization of ZnO NPs 2 2.7. Effect of L-ZnO, FL-ZnO and OFL-ZnO NPs on the planktonic growth of C. albicans 2 2.8. Determination of reactive oxygen species (ROS) 3 2.9. Morphogenesis inhibition assay 3 2.10. Biofilm inhibition assay 3 2.11. Gene expression analysis 3 2.12. In-vitro cell cytotoxicity study 3 2.13. In-vivo animal study 3 2.14. Statistics 4 3. Results 4 3.1. Characterization of lignin, FL, and OFL 4 3.2. Characterization of L-ZnO, FL-ZnO and OFL-ZnO NPs 4 3.3. Effect of L-ZnO, FL-ZnO and OFL-ZnO NPs on the planktonic growth of C. albicans 5 3.4. Morphogenesis inhibition 5 3.5. Biofilm inhibition 5 3.6. Gene expression analysis 5 3.7. Biocompatibility study of FL-ZnO NPs 6 4. Discussion 6 4.1. Characterization of lignin, FL, and OFL 7 4.2. Synthesis and characterization of L-ZnO, FL-ZnO and OFL-ZnO NP

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nonfiction