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Generation of Escherichia coli intimin derivatives with differing biological activities using site-directed mutagenesis of the intimin C-terminus domain by Gad Frankel; Alan D. Philips; Michaela Novakova; Miranda Batchelor; Susan Hicks; Gordon Dougan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Generation of Escherichia coli intimin derivatives with differing biological activities using site-directed mutagenesis of the intimin C-terminus domain about?

Intimins, encoded by __eae__ genes, are outer membrane proteins involved in attaching–effacing (A/E) lesion formation and host cell invasion by pathogenic bacteria, including enteropathogenic __Escherichia coli__ (EPEC) and __Citrobacter rodentium__. A series of intimins, harbouring specific mutations close to the C‐terminus, were constructed using pCVD438, which encodes the __eae__ gene from EPEC strain E2348/69. These mutant plasmids were introduced into EPEC strain CVD206 and __C. rodentium__ strain DBS255, which both contain deletion mutations in their __eae__ genes. CVD206, CVD206(pCVD438) and CVD206(pCVD438) derivatives were assessed for their ability to promote A/E lesion formation or invasion of HEp‐2 cells and to induce A/E lesions on fresh human intestinal __in vitro__ organ cultures (IVOC). The pathogenicity of __C. rodentium__ DBS255 harbouring these plasmid derivatives was also studied in mice. Here, we report that intimin‐mediated A/E lesion formation can be segregated from intimin‐mediated HEp‐2 cell invasion. Moreover, adherence to IVOC, EPEC‐induced microvillus elongation and colonization of the murine intestine by __C. rodentium__ were also modulated by the modifi

Who reads Generation of Escherichia coli intimin derivatives with differing biological activities using site-directed mutagenesis of the intimin C-terminus domain?

Author

Gad Frankel; Alan D. Philips; Michaela Novakova; Miranda Batchelor; Susan Hicks; Gordon Dougan

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)