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Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations by Antonino Baez; Noemí Flores; Francisco Bolívar; Octavio T. Ramírez is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The effect of dissolved carbon dioxide (dCO~2~) concentration on the stoichiometric and kinetic constants and by‐product accumulation was determined for __Escherichia coli__ cells producing recombinant green fluorescent protein (GFP). Constant dCO~2~, in the range of 20–300 mbar, was maintained during batch cultures by manipulating the inlet gas composition. As dCO~2~ increased, specific growth rate (__μ__) decreased, and acetate accumulation and the time for onset of GFP production increased. Maximum biomass yield on glucose and GFP concentration were affected for dCO~2~ above 70 and 150 mbar, respectively. Expression analysis of 16 representative genes showed that __E. coli__ can respond at the transcriptional level upon exposure to increasing dCO~2~, and revealed possible mechanisms responsible for the detrimental effects of high dCO~2~. Genes studied included those involved in decarboxylation reactions (__aceF__, __icdA__, __lpdA__, __sucA__, __sucB__), genes from pathways of production and consumption of acetate (__ackA__, __poxB__, __acs__, __aceA__, __fadR__), genes from gluconeogenic and anaplerotic metabolism (__pckA__, __ppc__), genes from the acid resistance

Who reads Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations?

Author

Antonino Baez; Noemí Flores; Francisco Bolívar; Octavio T. Ramírez

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)