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Quality evaluation of raw and processed Corni Fructus by UHPLC-QTOF-MS and HPLC coupled with color determination 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and reagents 2 2.2 Sample preparation 2 2.3 Instruments and conditions 2 2.3.1 HPLC system condition 2 2.3.2 UPLC-Q-TOF-MS system condition 2 2.3.3 Colorimeter and condition 2 2.4 Data analysis 2 3 Results and discussion 3 3.1 Quantitative analysis by HPLC 3 3.1.1 Optimization of extraction and chromatographic conditions 3 3.1.2 Methodology validation for quantitative analysis 3 3.1.3 Sample quantitative analysis 3 3.2 UHPLC-QTOF-MS analysis 3 3.2.1 Optimization of chromatographic and mass spectrometric condition 3 3.2.2 Identification of compounds by UHPLC-QTOF-MS 3 3.2.3 Principal components analysis (PCA) 6 3.2.4 Orthogonal Partial Least Squares-discriminant Analysis (OPLS-DA) 6 3.3 Color determination of samples 6 3.3.1 Optimization of color determination mode 6 3.3.2 Methodology validation for color determination 6 3.3.3 Global analysis of color parameters 6 3.3.4 Correlation analysis between color parameters and compounds 6 4 Conclusion 11 CRediT authorship contribution statement 11 Declaration of Competing

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Language

EN

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nonfiction