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Effect of Enzyme-assisted Extraction on the Physicochemical Properties and Bioactive Potential of Lotus Leaf Polysaccharides by Young-Ran Song; Ah-Ram Han; Seul-Gi Park; Chang-Won Cho; Young-Kyoung Rhee; Hee-Do Hong is a Materials Science article available to read on EtoBox.
What is Effect of Enzyme-assisted Extraction on the Physicochemical Properties and Bioactive Potential of Lotus Leaf Polysaccharides about?
Lotus leaf polysaccharides were extracted by enzyme-assisted extraction using α-amylase (LLEP-A), cellulose (LLEP-C), pectinase (LLEP-P) or protease (LLEP-PR). Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP). HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions. Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation. LLEP-P was further purified by gel filtration, and the main fraction (LLEP-P-І) was obtained to elucidate the structural and functional properties. LLEP-P-І (14.63 × 10 g/mol) mainly consisted of rhamnose, arabinose, galactose, and galacturonic acid at molar percentages of 15.5:15.8:20.1:32.8. FT-IR spectra indicated the predominant acidic and esterified form, suggesting the pectic-like structure. Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic
Who reads Effect of Enzyme-assisted Extraction on the Physicochemical Properties and Bioactive Potential of Lotus Leaf Polysaccharides?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Young-Ran Song; Ah-Ram Han; Seul-Gi Park; Chang-Won Cho; Young-Kyoung Rhee; Hee-Do Hong
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-8130)
- Published
- 2020
- Language
- EN
- Field
- Materials Science (Physical Sciences)