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Cholinesterase-based Dipstick Assay for the Detection of Organophosphate and Carbamate Pesticides by Hwa-Young No; Young Ah Kim; Yong Tae Lee; Hye-Sung Lee is a Chemistry article available to read on EtoBox.
What is Cholinesterase-based Dipstick Assay for the Detection of Organophosphate and Carbamate Pesticides about?
A cholinesterase (ChE)-based dipstick-type assay for the class-specific detection of organophosphate (OP) and carbamate (CM) pesticides was developed. The principle of the assay is based on inhibition of the activity of a ChE by these two families of pesticides, which is dependent on the concentration of pesticides. The proposed assay system is composed of a test strip with an acetylcholinesterase (AChE)-coated membrane and an enzyme substrate solution. The assay protocol involves incubation of the enzyme-coated strip in the pesticide-containing sample solution followed by incubation of the sample-treated strip in a chromogenic enzyme substrate solution. The color intensity is estimated by the naked eye or a reflectometer. Of the membranes tested as the enzyme support, Hybond N + was the most suitable. Among the compounds tested as the enzyme substrate, indophenyl acetate was the best. The detectable concentration range of the dipstick assay for the OP and CM pesticides was 10 -6 -10 2 and 10 -6 -10 0 g mL -1 , respectively. The sensitivity of the dipstick assay to the oxidized form of parathion (paraoxon) was higher than to parathion. The strip showed a large matrix effect with pe
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It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Hwa-Young No; Young Ah Kim; Yong Tae Lee; Hye-Sung Lee
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)