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Optimization and Design Considerations of Two-phase Continuous Protein Separation by E Huenupi; A Gomez; B A Andrews; J A Asenjo is a Engineering article available to read on EtoBox.

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Aqueous two-phase systems (ATPS) have begun to be used industrially for the isolation and separation of recombinant enzyme proteins. However, until now most studies have concentrated on investigating factors aþ ecting the partition coefficients of molecules and even the pilot plant and scale-up developments have lacked engineering, optimization and design considerations. This paper investigates such considerations in the process of protein separation and puriücation in ATPS. A cost function for the process of protein extraction that takes into account the eþ ect of variables in ATPS has been developed to study the inýuence of this extraction stage over the rest of the stages in the process (production, concentration and puriücation). The eþ ect of the ýow rate ratios, the relative sizes of the PEG and Phosphate phases and the NaCl concentration have been analysed. An extended mathematical model was used to describe the continuous, steady state operation of an aqueous twophase system for protein extraction. The basic model is based on steady state mass balances of the main components and phase equilibrium data. Experimental data on the separation of a-amylase from Bacillus subtilis

Who reads Optimization and Design Considerations of Two-phase Continuous Protein Separation?

Author

E Huenupi; A Gomez; B A Andrews; J A Asenjo

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0268-2575)

Published

1999

Language

EN

Field

Engineering (Physical Sciences)