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A Novel Triple-wavelength Colorimetric Method for Measuring Amylose and Amylopectin Contents by JinPeng Wang; Yin Li; YaoQi Tian; XueMing Xu; XueXia Ji; Xu Cao; ZhengYu Jin is a Nursing article available to read on EtoBox.

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## Abstract A novel triple‐wavelength colorimetry based on the principle of iodine binding was developed to determine amylose and amylopectin contents. Detection wavelengths of different starch material were different for each method. Comparative trials were carried out by both single‐ and dual‐wavelength colorimetry. We found that single‐wavelength colorimetry could only be used to evaluate amylose content. Correlation coefficient of standard curves for amylopectin content determination was improved by increase of detection wavelength numbers. The precision and recovery coefficient was also improved by triple‐wavelength colorimetry compared to single‐ and dual‐wavelength colorimetry. Percent amylose of cassava, potato, and high‐amylose maize determined by triple‐wavelength were 19.3, 21.8, and 42.3%, respectively, and percent amylopectin were 78.9, 76.9, and 54.5%, respectively. This developed triple‐wavelength colorimetry could provide accurate data for evaluating amylose content and have potential for amylopectin content detection.

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Author

JinPeng Wang; Yin Li; YaoQi Tian; XueMing Xu; XueXia Ji; Xu Cao; ZhengYu Jin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley; FASEB (ISSN 0038-9056)

Published

2010

Language

EN

Field

Nursing (Life Sciences)