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Can I read Biosynthesis of the Calorie-Free Sweetener Precursor ent -Kaurenoic Acid from CO 2 Using Engineered Cyanobacteria on EtoBox?
Biosynthesis of the Calorie-Free Sweetener Precursor ent -Kaurenoic Acid from CO 2 Using Engineered Cyanobacteria by Ko, Sung Cheon; Woo, Han Min is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Biosynthesis of the Calorie-Free Sweetener Precursor ent -Kaurenoic Acid from CO 2 Using Engineered Cyanobacteria about?
To supply the sustainable calorie-free sweetener stevioside, synthetic photosynthetic bacteria were developed to produce entkaurenoic acid as a precursor of stevioside directly from CO 2 . By the use of a combinatorial and modular approach for gene expression, including a cytochrome P450 and the corresponding reductase, engineered Synechoccous elongatus PCC 7942 as a model cyanobacterium enabled the biosynthesis of ent-kaurenoic acid at 2.9 ± 0.01 mg L -1 from CO 2 . We found that the order of genes for expression was critical, producing ent-kaurenoic acid by balancing gene expressions and accumulation of the toxic intermediate in a cell. The engineered bacteria allowed the complete biosynthesis of ent-kaurenoic acid, and it will be used for stevioside biosynthesis from CO 2 as a controlled fermentation.
Who reads Biosynthesis of the Calorie-Free Sweetener Precursor ent -Kaurenoic Acid from CO 2 Using Engineered Cyanobacteria?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ko, Sung Cheon; Woo, Han Min
- Publisher
- American Chemical Society; American Chemical Society (ACS) (ISSN 2161-5063)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)