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Biosynthesis of Cholestanol in Higher Plants by Naoko Nakajima; Shozo Fujioka; Takashi Tanaka; Suguru Takatsuto; Shigeo Yoshida is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Biosynthesis of Cholestanol in Higher Plants about?

To understand the early steps of C 27 brassinosteroid biosynthesis, metabolic experiments were performed with Arabidopsis thaliana and Nicotiana tabacum seedlings, and with cultured Catharanthus roseus cells. [26, 28-2 H 6 ]Campestanol, [26-2 H 3 ]cholesterol, and [26-2 H 3 ]cholestanol were administered to each plant, and the resulting metabolites were analyzed by gas chromatography-mass spectrometry. In all the species examined, [ 2 H 3 ]cholestanol was identified as a metabolite of [ 2 H 6 ]campestanol, and [ 2 H 3 ]cholest-4-en-3one and [ 2 H 3 ]cholestanol were identified as metabolites of [ 2 H 3 ]cholesterol. This study revealed that cholestanol (C 27 sterol) was biosynthesized from both cholesterol (C 27 sterol) and campestanol (C 28 sterol). It was also demonstrated that cholestanol was converted to 6-oxocholestanol, and campestanol was converted to 6-oxocampestanol.

Who reads Biosynthesis of Cholestanol in Higher Plants?

Author

Naoko Nakajima; Shozo Fujioka; Takashi Tanaka; Suguru Takatsuto; Shigeo Yoshida

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)