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Amino acid sequence of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit from Euglena by Alain Sailland; Iradj Amiri; Georges Freyssinet is a Agricultural and Biological Sciences article available to read on EtoBox.

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The amino acid sequence of the ribulose-l,5-bisphosphate carboxylase/oxygenase (RuBisCO) small subunit (SSU) from Euglena has been established by alignment of the sequence of peptides obtained by cleavage with chymotrypsin, trypsin, Staphylococcus aureus protease or formic acid. The Euglena SSU has 138 amino acids and thus represents longest SSU sequence described so far. Homology is only 41070 with cyanobacteria SSU and about 51070 with higher plant SSU, whereas it is around 75°7o between higher plants. The largest homologous portion between all the known SSU sequences is localized in the second half and covers about 20 amino acids. The phylogenetic tree based on known SSU sequences has been established and the rate of amino acid substitution for SSU is estimated to be about 1.35 x 10 -9 per year and per site. Despite heterogeneity in amino acid sequence, we found that the overall secondary structure is fairly well conserved.

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Author

Alain Sailland; Iradj Amiri; Georges Freyssinet

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1986

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)