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DNA adduct formation by the environmental contaminant 3‐nitrobenzanthrone after intratracheal instillation in rats by Christian A. Bieler; Michael G. Cornelius; Reinhold Klein; Volker M. Arlt; Manfred Wiessler; David H. Phillips; Heinz H. Schmeiser is a Medicine article available to read on EtoBox.
What is DNA adduct formation by the environmental contaminant 3‐nitrobenzanthrone after intratracheal instillation in rats about?
## Abstract 3‐Nitrobenzanthrone (3‐NBA) is an environmental pollutant and suspected human carcinogen found in emissions from diesel and gasoline engines and on the surface of ambient air particulate matter; human exposure to 3‐NBA is likely to occur primarily via the respiratory tract. In our study female Sprague Dawley rats were treated by intratracheal instillation with a single dose of 0.2 or 2 mg/kg body weight of 3‐NBA. Using the butanol enrichment version of the ^32^P‐postlabeling method, DNA adduct formation by 3‐NBA 48 hr after intratracheal administration in different organs (lung, pancreas, kidney, urinary bladder, heart, small intestine and liver) and in blood was investigated. The same adduct pattern consisting of up to 5 DNA adduct spots was detected by thin layer chromatography in all tissues and blood and at both doses. Highest total adduct levels were found in lung and pancreas (350 ± 139 and 620 ± 370 adducts per 10^8^ nucleotides for the high dose and 39 ± 18 and 55 ± 34 adducts per 10^8^ nucleotides for the low dose, respectively) followed by kidney, urinary bladder, heart, small intestine and liver. Adduct levels were dose‐dependent in all organs (approximately
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- Author
- Christian A. Bieler; Michael G. Cornelius; Reinhold Klein; Volker M. Arlt; Manfred Wiessler; David H. Phillips; Heinz H. Schmeiser
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)