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Enhanced Chondrocyte Proliferation and Mesenchymal Stromal Cells Chondrogenesis in Coculture Pellets Mediate Improved Cartilage Formation by Chitrangada Acharya; Adetola Adesida; Paul Zajac; Marcus Mumme; Jens Riesle; Ivan Martin; Andrea Barbero is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract In this study, we aimed at investigating the interactions between primary chondrocytes and mesenchymal stem/stromal cells (MSC) accounting for improved chondrogenesis in coculture systems. Expanded MSC from human bone marrow (BM‐MSC) or adipose tissue (AT‐MSC) were cultured in pellets alone (monoculture) or with primary human chondrocytes from articular (AC) or nasal (NC) cartilage (coculture). In order to determine the reached cell number and phenotype, selected pellets were generated by combining: (i) human BM‐MSC with bovine AC, (ii) BM‐MSC from HLA‐A2+ with AC from HLA‐A2− donors, or (iii) human green fluorescent protein transduced BM‐MSC with AC. Human BM‐MSC and AC were also cultured separately in transwells. Resulting tissues and/or isolated cells were assessed immunohistologically, biochemically, cytofluorimetrically, and by RT‐PCR. Coculture of NC or AC (25%) with BM‐MSC or AT‐MSC (75%) in pellets resulted in up to 1.6‐fold higher glycosaminoglycan content than what would be expected based on the relative percentages of the different cell types. This effect was not observed in the transwell model. BM‐MSC decreased in number (about fivefold) over time and, if co
Who reads Enhanced Chondrocyte Proliferation and Mesenchymal Stromal Cells Chondrogenesis in Coculture Pellets Mediate Improved Cartilage Formation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Chitrangada Acharya; Adetola Adesida; Paul Zajac; Marcus Mumme; Jens Riesle; Ivan Martin; Andrea Barbero
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)