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Clinical significance of ESR1 gene copy number changes in breast cancer as measured by fluorescence in situ hybridisation by Ching-Hung Lin; Jacqueline M Liu; Yen-Shen Lu; Chieh Lan; Wei-Chung Lee; Kuan-Ting Kuo; Chung-Chieh Wang; Dwan-Ying Chang; Chiun-Sheng Huang; Ann-Lii Cheng is a Medicine article available to read on EtoBox.
What is Clinical significance of ESR1 gene copy number changes in breast cancer as measured by fluorescence in situ hybridisation about?
## Aims The__ESR1__gene encodes for oestrogen receptor (ER) α, which plays a crucial role in mammary carcinogenesis and clinical outcome in patients with breast cancer. However, the clinical significance of the__ESR1__gene copy number change for breast cancer has not been clarified. ## Methods __ESR1__gene copy number was determined by fluorescence in situ hybridisation (FISH) on tissue sections. A minimum of 20 tumour cells were counted per section, and a FISH ratio of__ESR1__gene to CEP6 ≥2.0 was considered__ESR1__amplification. A ratio >1.2 but <2.0 was considered__ESR1__gain. The__ESR1__copy number was further measured by quantitative real-time PCR (Q-PCR) with ASXL2 as a reference. ## Results FISH revealed__ESR1__amplification in six cases (4.0%) and__ESR1__gain in 13 cases (8.7%) from a total of 150 cases.__ESR1__gain and amplification were more common in older patients (p<0.001), and correlated well with ER protein expression (p=0.03) measured by immunohistochemistry, and__ESR1__copy number (p<0.001) measured by Q-PCR. Furthermore, the multivariate analysis revealed that__ESR1__amplification was associated with a shorter disease-free survival (HR=5.56, p=0.03) and a shorter
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- Author
- Ching-Hung Lin; Jacqueline M Liu; Yen-Shen Lu; Chieh Lan; Wei-Chung Lee; Kuan-Ting Kuo; Chung-Chieh Wang; Dwan-Ying Chang; Chiun-Sheng Huang; Ann-Lii Cheng
- Publisher
- BMJ
- Published
- 2012
- Language
- EN
- Field
- Medicine (Health Sciences)