Can I read Nanoparticle-assisted pyrrolidonyl arylamidase assay for a culture-free Group A Streptococcus pyogenes detection with image analysis on EtoBox?
Nanoparticle-assisted pyrrolidonyl arylamidase assay for a culture-free Group A Streptococcus pyogenes detection with image analysis by Merve Eryılmaz; Uğur Tamer; İsmail Hakkı Boyacı is a Chemistry article available to read on EtoBox.
What is Nanoparticle-assisted pyrrolidonyl arylamidase assay for a culture-free Group A Streptococcus pyogenes detection with image analysis about?
Existing techniques for the detection of Group A Streptococcus pyogenes (GAS) have drawbacks in rapidness, accuracy or in high-cost. Considering the clinical importance of GAS, we have developed a culture-free detection method based on pyrrolidonyl arylamidase (PYR) activity with the aid of magnetic gold nanoparticles (AuNPs). GAS is the reason for pharyngitis and sampling starts from the throat with cotton swabs. After swab sampling, the target was collected with antibody modified magnetic AuNPs and transferred into 500 μL of PYR-broth without any antigen extraction or pure colony isolation. Then, the assay was finished by adding 25 μL of 4-(dimethylamino)-cinnamaldehyde (DMACA) reagent after 4-h incubation. A red color formation was evaluated as the presence of GAS comparing to blank, however, image analysis was employed for the interpretation of color changes clearly. For this purpose, a formula related to image data was proposed and analytical validation parameters were defined. Thus, the correlation was found to be linear with the R of 0.9685 between the log of bacteria concentration and the image data with the limit of detection of 3.3 × 10 CFU/mL of GAS. In addition, the ass
Who reads Nanoparticle-assisted pyrrolidonyl arylamidase assay for a culture-free Group A Streptococcus pyogenes detection with image analysis?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Merve Eryılmaz; Uğur Tamer; İsmail Hakkı Boyacı
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0039-9140)
- Published
- 2020
- Language
- EN
- Field
- Chemistry (Physical Sciences)