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In vitro and in vivo processing studies of peptide antigens in context of MHC class II presentation by H. Kalbacher; H. Beck; C. Schröter; M. Deeg; T. Halder is a Immunology and Microbiology article available to read on EtoBox.

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## Introduction: We have observed that CD4+ T cell clones, elicited after infection of C57BlllO mice with influenza virus, which are specific for the haemagglutinin pepttde HA1 186-205 are able to respond to peptide in the absence of APC as measured by IL-3 release, but are unable to proliferate. The purpose of this study was to determine whether or not MHC class II plays a role in this response. Materials and Methods: Flow cytometry and cell sorting were used to study the induction and expression of surface MHC class II on T cell clones. R.T./PCR techniques were employed to study the expression of class II mRNA. Reauftaz We have shown that murtne CD4+ T cell clones can express ctass II molecules on their surface. We have shown that viral peptide, in the absence of APC induces de novo synthesis and expression of class II IAb. This induction is peptidespectftcand can be inhibited by TCR-specific monoclonal antibodies. FAGS-sorted CD4+ class II-were shown to express dass II mRNA by R.T./PCR, and these cells became class II+ when cultured with peptkte. Conduslon: We speculate that peptide specific induction of MHC class II expression may be a feature of Thl-type clones elicited by nat

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Author

H. Kalbacher; H. Beck; C. Schröter; M. Deeg; T. Halder

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)

Published

1997

Language

EN

Field

Immunology and Microbiology (Life Sciences)