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Ultrastructural morphology, synaptic relationships, and CGRP immunoreactivity of physiologically identified c‐fiber terminals in the monkey spinal cord by Dr. Francisco J. Alvarez; Anahid M. Kavookjian; Dr. Alan R. Light is a Neuroscience article available to read on EtoBox.

What is Ultrastructural morphology, synaptic relationships, and CGRP immunoreactivity of physiologically identified c‐fiber terminals in the monkey spinal cord about?

The spinal cord terminations of two electrophysiologically identified single C-fibers (one identified as a C-nociceptor) were intraaxonally labeled with horseradish peroxidase and analyzed with both light and electron microscopy. Serial section ultrastructural analysis and postembedding immunocytochemical techniques for calcitonin gene-related peptide (CGRP), substance P (SP), and GABA were used to study the synaptology, and neuropeptide content. All C-terminal synapses were in laminae I and II. The terminals sampled (n = 73) from these two C-fibers rarely established glomerular synaptic complexes, but rather, simple terminals, usually measuring 1-4 km in length and 1-3 km in diameter. They most often established 1 or 2 (range 1 tn 5 ) quite large asymmetric axodendritic synaptic contacts. Postsynaptic structures included dendritic spines and shafts with and without vesicles. C-terminals were filled with small round synaptic vesicles (45-60 nm) and also contained variable numbers of large dense-core vesicles (LDCVs, 80-1 10 nm). LDCVs inside identified C-terminals frequently displayed CGRP immunoreactivity. We were unable to detect SP immunoreactivity inside our sample of C-fiber L

Who reads Ultrastructural morphology, synaptic relationships, and CGRP immunoreactivity of physiologically identified c‐fiber terminals in the monkey spinal cord?

Author

Dr. Francisco J. Alvarez; Anahid M. Kavookjian; Dr. Alan R. Light

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

1993

Language

EN

Field

Neuroscience (Life Sciences)