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Can I read Isoquercitrin protects HUVECs against high glucose-induced apoptosis through regulating p53 proteasomal degradation on EtoBox?
Isoquercitrin protects HUVECs against high glucose-induced apoptosis through regulating p53 proteasomal degradation by Libo Liu; Sihui Huang; Man Xu; Yan Gong; Dan Li; Chunxia Wan; Haiming Wu; Qizhu Tang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Isoquercitrin protects HUVECs against high glucose-induced apoptosis through regulating p53 proteasomal degradation about?
High glucose (HG)-induced endothelial apoptosis serves an important role in the vascular dysfunction associated with diabetes mellitus (DM). It has been reported that isoquercitrin (IQC), a flavonoid glucoside, possesses an anti-DM effect, but the mechanism requires further investigation. The present study investigated the effect of IQC against HG-induced apoptosis in human umbilical vein endothelial cells (HUVECs) and explored its molecular mechanism. HUVECs were treated with 5 or 30 mM glucose for 48 h. Endothelial cell viability was monitored using the Cell Counting Kit-8 assay. Mitochondrial membrane potential was detected by JC-1 staining. Apoptosis was observed by TUNEL staining and flow cytometry. Western blotting was used for the analysis of apoptosis-associated proteins Bax, Bcl-2, cleaved (C)-caspase3, total-caspase3, p53 and phosphorylated p53. Reverse transcription-quantitative PCR was used to analyze the mRNA expression levels of Bax, Bcl-2 and p53. Immunofluorescence staining was utilized to detect the expression levels and distribution of p53 and ubiquitin specific peptidase 10 (USP10) in HUVECs. The results revealed that IQC significantly attenuated HG-induced endot
Who reads Isoquercitrin protects HUVECs against high glucose-induced apoptosis through regulating p53 proteasomal degradation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Libo Liu; Sihui Huang; Man Xu; Yan Gong; Dan Li; Chunxia Wan; Haiming Wu; Qizhu Tang
- Publisher
- D.A. Spandidos
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)