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This document provides guidance on designing PCR primers through a series of practical exercises. It introduces PCR primer design software that is freely available for local installation. The purpose is to design primers for sequencing, amplification or detection of single nucleotide polymorphisms. Materials needed include primer design software like Primer3 and tools to check primer specificity. A step-by-step method is provided to use Primer3Plus to design a primer pair to amplify a DNA fragment between 4

Author

Nguyễn Minh Phương Uyên

Language

EN