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1X PBS Buffer Preparation Guide by Nguyễn Hoài Thu is a document available to read on EtoBox.
What is 1X PBS Buffer Preparation Guide about?
This document provides a recipe for making 1X Phosphate Buffered Saline (PBS Buffer) which is commonly used in biological research and applications. The recipe calls for dissolving 8g sodium chloride, 0.2g potassium chloride, 1.44g sodium phosphate dibasic, and 0.24g potassium phosphate monobasic in 800ml distilled water. The pH is then adjusted to 7.4 and the volume brought up to 1 liter with additional distilled water before sterilizing by autoclaving. PBS buffer can be made as either a 1X solution or con
- Author
- Nguyễn Hoài Thu
- Language
- EN