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Synthesis of poly(Pro–Hyp–Gly)n by direct polycondensation of (Pro–Hyp–Gly)n, where n = 1, 5, and 10, and stability of the triple-helical structure by Takahiro Kishimoto; Yasushi Morihara; Michinori Osanai; Shin-ichi Ogata; Masanobu Kamitakahara; Chikara Ohtsuki; Masao Tanihara is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Pro–Hyp–Gly is a characteristic amino acid sequence found in fibrous collagens, and (Pro–Hyp–Gly)~10~, which has been widely used as a collagen‐model peptide, forms a stable triple‐helical structure. Here, we synthesized polypeptides consisting of the Pro–Hyp–Gly sequence by direct polycondensation of (Pro–Hyp–Gly)~__n__~, where __n__ = 1, 5, and 10, using 1‐hydroxybenzotriazole and 1‐ethyl‐3‐(3‐dimethyl‐aminopropyl)‐carbodiimide hydrochloride in both phosphate buffer (pH = 7.4) and dimethylsulfoxide (DMSO) solutions for 48 h at 20°C. The reaction of (Pro–Hyp–Gly)~5~ and (Pro–Hyp–Gly)~10~ in DMSO successfully gave polypeptides with molecular weights over 10,000, whereas low molecular weight products were obtained by reaction in phosphate buffer (pH = 7.4). In contrast, Pro–Hyp–Gly at a concentration of 50 mg/mL in phosphate buffer (pH = 7.4) gave polypeptides with molecular weights over 10,000. The Fourier transform infrared (FTIR) and ^1^H nuclear magnetic resonance (NMR) spectra of poly(Pro–Hyp–Gly)~10~ revealed that the polymerization of (Pro–Hyp–Gly)~10~ described in this report had no side reactions. Each polypeptide obtained shows a collagen‐like triple‐helical st
Who reads Synthesis of poly(Pro–Hyp–Gly)n by direct polycondensation of (Pro–Hyp–Gly)n, where n = 1, 5, and 10, and stability of the triple-helical structure?
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- Author
- Takahiro Kishimoto; Yasushi Morihara; Michinori Osanai; Shin-ichi Ogata; Masanobu Kamitakahara; Chikara Ohtsuki; Masao Tanihara
- Publisher
- Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)