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Can I read miR-211-5p alleviates focal cerebral ischemia-reperfusion injury in rats by down-regulating the expression of COX2 on EtoBox?
miR-211-5p alleviates focal cerebral ischemia-reperfusion injury in rats by down-regulating the expression of COX2 by Peng, Zhe; Li, Miaomiao; Tan, Xiaodan; Xiang, Pu; Wang, Hong; Luo, Ying; Yang, Yang; Huang, Haifeng; Chen, Zhihao; Xia, Hui; Li, Yuke; Zhang, Jiahua; Gu, Chao; Liu, Maozhu; Wang, Qiong; Chen, Mengyuan; Yang, Junqing is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is miR-211-5p alleviates focal cerebral ischemia-reperfusion injury in rats by down-regulating the expression of COX2 about?
The present study was to investigate the role of microRNA (miR)-211-5p on cerebral ischemia-reperfusion injury (CIRI) and clarify its underlying mechanisms. Middle cerebral artery occlusion/reperfusion (MCAO/R) was operated on male Sprague Dawley (SD) rats, oxygen-glucose deprivation/reperfusion (OGD/R) was conducted on pheochromocytoma-12 (PC12) cells. Here, we found that miR-211-5p and Cyclooxygenase (COX2) expressions were altered in the plasma, cortex and hippocampus of MCAO/R-treated rats, as well as in the OGD/R-treaded PC12 cells. In vivo, overexpression of miR-211-5p resulted in decrease of infarct volumes, neurological deficit scores and histopathological damage. In vitro, miR-211-5p overexpression significantly decreased cell apoptosis and Lactate dehydrogenase (LDH) release rate, increased cell viability. Furthermore, our data showed that miR-211-5p overexpression markedly reduced the expressions of COX2 mRNA and protein, and the contents of Prostaglandin D2 (PGD2), PGE2, tumor necrosis factor-α (TNF-α) and Interleukin-1β (IL-1β). In addition, inhibition of COX2 significantly rescued the effects of miR-211-5p inhibitor. At last, dual luciferase experimental data showed t
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- Author
- Peng, Zhe; Li, Miaomiao; Tan, Xiaodan; Xiang, Pu; Wang, Hong; Luo, Ying; Yang, Yang; Huang, Haifeng; Chen, Zhihao; Xia, Hui; Li, Yuke; Zhang, Jiahua; Gu, Chao; Liu, Maozhu; Wang, Qiong; Chen, Mengyuan; Yang, Junqing
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0006-2952)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)