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A Multicentre Assessment of the Endogenous Thrombin Potential Using a Continuous Monitoring Amidolytic Technique by Anorew S. Lawrie; Elaine Gray; Dave Leeming; Simon J. Davidson; Gordon Purdy; Romina Iampietro; Steve Craig; Peter Rigsby; Ian J. Mackie is a Medicine article available to read on EtoBox.

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**Summary.** This study assessed the inter‐laboratory imprecision associated with the measurement of the endogenous thrombin potential (ETP). The initial studies used techniques that had evolved in each of the participating laboratories. Samples from normal healthy subjects (__n__ = 10), two patients receiving coumarin therapy [International Normalized Ratio ∼2·0 and ∼4·0] and a further two subjects receiving treatment with unfractionated heparin (anti‐Xa 0·07 iu/ml and 0·31 iu/ml) were assayed relative to a lyophilized normal plasma that had arbitrarily been assigned a potency of 100%. Considerable variation in potency estimates was observed between the centres, although individual laboratories using fully automated techniques achieved acceptable levels of imprecision as assessed by the coefficient of variation (CV) (intra‐assay CV < 9·5%, inter‐assay CV 0·96) between the chromogenic substrates. Our data demonstrates that an automated ETP method can be standardized between laboratories and suitable levels of imprecision achieved, using different analysers (COBAS Mira at two centres and an ACL‐300R) and two thrombin substrates. This indicates that more widespread use of ETP measu

Who reads A Multicentre Assessment of the Endogenous Thrombin Potential Using a Continuous Monitoring Amidolytic Technique?

Author

Anorew S. Lawrie; Elaine Gray; Dave Leeming; Simon J. Davidson; Gordon Purdy; Romina Iampietro; Steve Craig; Peter Rigsby; Ian J. Mackie

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

2003

Language

EN

Field

Medicine (Health Sciences)