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Flow cytometric analysis of Saccharomyces cerevisiae autolytic mutants and protoplasts by J. M. de la Fuente; C. Nombela; M. Sanchez; A. Alvarez is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Flow cytometric analysis of Saccharomyces cerevisiae autolytic mutants and protoplasts about?

Simple methods, based on the technique of flow cytometry, have been developed for the phenotypic characterization of yeast autolytic mutants and for the analysis of the formation and regeneration of the yeast protoplasts. The expression of lytic mutations determined uptake of the fluorescent dye propidium iodide, which could be carefully monitored by flow cytometry. Mixed populations of lysed and viable cells were precisely quantified and sorted, and the technique was also applied to demonstrate protection from lysis of mutant cells with cell wail defects, in the presence of osmotic stabilizers. Protoplast formation and regeneration was monitored by analysing relative cell size; this was facilitated by the preparation of homogeneous protoplast preparations. The technique of flow cytometry proved superior to other conventional methods for these types of study.

Who reads Flow cytometric analysis of Saccharomyces cerevisiae autolytic mutants and protoplasts?

Author

J. M. de la Fuente; C. Nombela; M. Sanchez; A. Alvarez

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)