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Can I read A village in a dish model system for population-scale hiPSC studies on EtoBox?
A village in a dish model system for population-scale hiPSC studies by Drew R. Neavin; Angela M. Steinmann; Nona Farbehi; Han Sheng Chiu; Maciej S. Daniszewski; Himanshi Arora; Yasmin Bermudez; Cátia Moutinho; Chia-Ling Chan; Monique Bax; Mubarika Tyebally; Vikkitharan Gnanasambandapillai; Chuan E. Lam; Uyen Nguyen; Damián Hernández; Grace E. Lidgerwood; Robert M. Graham; Alex W. Hewitt; Alice Pébay; Nathan J. Palpant; Joseph E. Powell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A village in a dish model system for population-scale hiPSC studies about?
The mechanisms by which DNA alleles contribute to disease risk, drug response, and other human phenotypes are highly context-specific, varying across cell types and different conditions. Human induced pluripotent stem cells are uniquely suited to study these context-dependent effects but cell lines from hundreds or thousands of individuals are required. Village cultures, where multiple induced pluripotent stem lines are cultured and differentiated in a single dish, provide an elegant solution for scaling induced pluripotent stem experiments to the necessary sample sizes required for population-scale studies. Here, we show the utility of village models, demonstrating how cells can be assigned to an induced pluripotent stem line using single-cell sequencing and illustrating that the genetic, epigenetic or induced pluripotent stem line-specific effects explain a large percentage of gene expression variation for many genes. We demonstrate that village methods can effectively detect induced pluripotent stem line-specific effects, including sensitive dynamics of cell states.
Who reads A village in a dish model system for population-scale hiPSC studies?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Drew R. Neavin; Angela M. Steinmann; Nona Farbehi; Han Sheng Chiu; Maciej S. Daniszewski; Himanshi Arora; Yasmin Bermudez; Cátia Moutinho; Chia-Ling Chan; Monique Bax; Mubarika Tyebally; Vikkitharan Gnanasambandapillai; Chuan E. Lam; Uyen Nguyen; Damián Hernández; Grace E. Lidgerwood; Robert M. Graham; Alex W. Hewitt; Alice Pébay; Nathan J. Palpant; Joseph E. Powell
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)