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1,1-Dimethylallylcoumarins potently suppress both lipopolysaccharide- and interferon-gamma-induced nitric oxide generation in mouse macrophage RAW 264.7 cells by Akira Murakami; Guanxin Gao; Mitsuo Omura; Masamichi Yano; Chihiro Ito; Hiroshi Furukawa; Daisuke Takahashi; Koichi Koshimizu; Hajime Ohigashi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is 1,1-Dimethylallylcoumarins potently suppress both lipopolysaccharide- and interferon-gamma-induced nitric oxide generation in mouse macrophage RAW 264.7 cells about?

We investigated the suppressive effects of 16 coumarin-related compounds on both lipopolysaccharide (LPS)- and interferon (IFN)-gamma-induced nitric oxide (NO) generation in a mouse macrophage cell line, RAW 264.7. Notably, coumarins possessing prenyl unit(s) were found to be highly active, a tendency consistent with our previous study. Among the coumarins tested, 1,1-dimethylallylcoumarins showed the highest inhibitory activity. Western blotting analysis revealed that they inhibited NO generation by suppressing inducible NO synthase (iNOS) protein expression. Our ongoing studies suggest that coumarins are prominent natural compounds that attenuate excessive and prolonged NO generation at inflammatory sites.

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Author

Akira Murakami; Guanxin Gao; Mitsuo Omura; Masamichi Yano; Chihiro Ito; Hiroshi Furukawa; Daisuke Takahashi; Koichi Koshimizu; Hajime Ohigashi

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0960-894X)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)