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The Degree of Starch Phosphorylation Is Related to the Chain Length Distribution of the Neutral and the Phosphorylated Chains of Amylopectin by Andreas Blennow; Anne Mette Bay-Smidt; Bente Wischmann; Carl Erik Olsen; Birger Lindberg Møller is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The Degree of Starch Phosphorylation Is Related to the Chain Length Distribution of the Neutral and the Phosphorylated Chains of Amylopectin about?
The chain length distribution of the amylopectin neutral and phosphorylated -glucan chains of isoamylase (EC 3.2.1.68)-debranched starch in dependence of starch phosphorylation has been analyzed using high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Neutral chains of moderately phosphorylated starches showed polymodal distributions with main peaks at dp (degree of polymerisation) 14±15 and 50, respectively. Highly phosphorylated starches speci®cally showed an increasing proportion of chains with mean dp 19. In the extremely highly phosphorylated curcuma (Curcuma spp.) starch, this chain population dominated the chain length pro®le. The sub-population of the corresponding phosphorylated chains from highly phosphorylated starches showed longer retention times than moderately phosphorylated starches on HPAEC. Separation of the phosphorylated chains using anionexchange chromatography at neutral pH demonstrated a partial separation of chains containing Glc3P residues and Glc6P residues, respectively, as well as a separation of chains containing one or several phosphate groups. Major determinants for the observed elution characteristics of ph
Who reads The Degree of Starch Phosphorylation Is Related to the Chain Length Distribution of the Neutral and the Phosphorylated Chains of Amylopectin?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Andreas Blennow; Anne Mette Bay-Smidt; Bente Wischmann; Carl Erik Olsen; Birger Lindberg Møller
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)