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Polyamines as Biomarkers of Cervical Intraepithelial Neoplasia by Kenji Nishioka; Alejandro B. Melgarejo; Rosanna R. Lyon; Michele Follen Mitchell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Polyamines as Biomarkers of Cervical Intraepithelial Neoplasia about?

Polyamines (putrescine, spermidine and spermine) play critical roles in cell growth and transformation. Ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, is considered a putative protooncogene crucial to the regulation of cell growth and transformation. Cancer patients have elevated levels of polyamines in their physiological fluids compared to normal counterparts. a-Difluoromethylomithine (DFMO), a specific suicide inhibitor of O X , exhibits antitumor and antimetastasis activities, and displays effectiveness in many carcinogen-induced animal chemoprevention models. Therefore, we are using DFMO in a chemoprevention trial for cervical intraepithelial neoplasia grade 111 (CIN III), and evaluating patients for changes in polyamine metabolism as an intermediate marker of DFMO effect. A preliminary study showed that several milligrams of abnormal cervical biopsy tissue contained detectable levels of ODC activity and polyamines. Additionally, the presence of cadaverine suggested bacterial contamination of these tissues. For this reason, normal and abnormal biopsies collected during colposcopy were rinsed prior to frozen storage. In most patients, abnormal tissues sh

Who reads Polyamines as Biomarkers of Cervical Intraepithelial Neoplasia?

Author

Kenji Nishioka; Alejandro B. Melgarejo; Rosanna R. Lyon; Michele Follen Mitchell

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)