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Initial Characterization of the Nascent Polypeptide-associated Complex in Yeast by Reimann, Barbara; Bradsher, John; Franke, Jacqueline; Hartmann, Enno; Wiedmann, Martin; Prehn, Siegfried; Wiedmann, Brigitte is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Initial Characterization of the Nascent Polypeptide-associated Complex in Yeast about?

The three subunits of the nascent polypeptide-associated complex ( , 1 , 3 ) in Saccharomyces cerevisiae are encoded by three genes (EGD2, EGD1, BTT1). We found the complex bound to ribosomes via the -subunits in a salt-sensitive manner, in close proximity to nascent polypeptides. Estimation of the molecular weight of the complex of wild-type cells and cells lacking one or two subunits revealed that the composition of the complex is variable and that as yet unknown proteins might be included. Regardless of the variability, a certain balance of the subunits has to be maintained: the deletion of one subunit causes downregulation of the remaining subunits at physiological growth temperature. Cells lacking both -subunits are unable to grow at 37 C, most likely due to a toxic effect of the -subunit. Based on in vitro experiments, it has been proposed that the function of mammalian nascent-polypeptide associated complexes (NAC) is to prevent inappropriate targeting of non-secretory nascent polypeptides. In vivo, however, the lack of NAC does not cause secretion of signal-less invertase in yeast. This result and the lack of a drastic phenotype of cells missing one, two or three subunits a

Who reads Initial Characterization of the Nascent Polypeptide-associated Complex in Yeast?

Author

Reimann, Barbara; Bradsher, John; Franke, Jacqueline; Hartmann, Enno; Wiedmann, Martin; Prehn, Siegfried; Wiedmann, Brigitte

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)