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Anti-neuroblastoma antibody chCE7 binds to an isoform of L1-CAM present in renal carcinoma cells by Marina L. Meli; François Carrel; Robert Waibel; Hanspeter Amstutz; Nigel Crompton; Rolf Jaussi; Holger Moch; P. August Schubiger; Ilse Novak-Hofer is a Medicine article available to read on EtoBox.
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Immunoprecipitation after cell surface labeling of human neuroblastoma cells showed that the anti-neuroblastoma monoclonal antibody (mAb) chCE7 binds to a 200,000 M r cell surface protein. The protein was partially purified by immunoaffinity chromatography from a human renal carcinoma and a human neuroblastoma cell line, which both showed high levels of binding of MAb chCE7. NH 2 -terminal sequences of 18 and 15 amino acid residues were determined. Both sequences isolated from the renal carcinoma and the neuroblastoma cells showed strong homology to human cell adhesion molecule L1 (L1-CAM), and both were characterized by the NH 2 -terminal deletion of 5 amino acids, comprising exon 2 of L1-CAM. Reverse trancription-polymerase chain reaction (RT-PCR) analysis of the regions spanning exon 2 and exon 27 of L1-CAM indicated that in neuroblastoma cells both transcripts for the full-length and exon-deleted forms are present, whereas in the renal carcinoma cell lines only the exon-deleted L1-CAM isoform were detected. Western blot analysis showed that 6 of 7 tested renal carcinoma cell lines and 5 of 15 renal carcinoma tissues expressed L1-CAM. In normal adult kidney tissue, very low leve
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- Author
- Marina L. Meli; François Carrel; Robert Waibel; Hanspeter Amstutz; Nigel Crompton; Rolf Jaussi; Holger Moch; P. August Schubiger; Ilse Novak-Hofer
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1999
- Language
- EN
- Field
- Medicine (Health Sciences)