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Overproduction of 3-Hydroxypropionate in a Super Yeast Chassis by Wei Yu; Xuan Cao; Jiaoqi Gao; Yongjin J. Zhou is a Engineering article available to read on EtoBox.

What is Overproduction of 3-Hydroxypropionate in a Super Yeast Chassis about?

3-Hydroxypropionate (3-HP) is a platform chemical for production of acrylic acid, acrylamide and biodegradable polymers. Several microbial cell factories have been constructed for production of 3-HP from malonyl-CoA by using a malonyl-CoA reductase, which however suffer from inadequate supply of precursor and cofactor. Here 3-HP biosynthesis was optimized in a super yeast chassis with sufficient supply of precursor malonyl-CoA and cofactor NADPH, which had a 3-fold higher 3-HP (1.4 g/L) than that of wild-type background. The instability of the engineered strain was observed in fed-batch fermentation due to the plasmid loss, which may be caused by the toxic intermediate malonate semialdehyde. Genome integration of MCR-C encoding C-terminal of MCR enabled stable gene expression and much higher 3-HP production of 4.4 g/L under batch fermentation and 56.5 g/L under fed-batch fermentation with a yield of 0.31 g/g glucose. This was the highest 3-HP production reported from glucose in engineered microbes.

Who reads Overproduction of 3-Hydroxypropionate in a Super Yeast Chassis?

Author

Wei Yu; Xuan Cao; Jiaoqi Gao; Yongjin J. Zhou

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Engineering (Physical Sciences)