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Fluorescent labeling and tracing of immobilized efficient degrading bacterium DNB-S1 and its remediation efficiency of DBP contaminated soil by Lei Wang; Xiaochen Jia; Zeyu Dou; Xiaoqian Li; Wenjing Bao; Chaoran Ma; Hongye Wang; Lei Wang; Maofeng Dong; Ying Zhang is a Environmental Science article available to read on EtoBox.

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Dibutyl phthalate (DBP) is an organic pollutant frequently detected in soil, and is a reproductive poison that harms animals both before and after birth and has mutagenic, teratogenic, and carcinogenic effects. DBP removal from farmland has been the subject of extensive research in recent years. Efficient DBP degrading bacterial strains were screened in the laboratory. GFP (Green fluorescent protein) labeled degradation strain GFP-DNB-S1 was analyzed for its activity and dynamics. Using sodium alginate (SA) and nano-hydroxyapatite (n-HAP) as carrier materials and CaCl2 as a cross-linking agent, the immobilized microbial agent n-HAP/SA + DNB-S1 was prepared by embedding cross-linking immobilization technology to study the remediation effect of DBP contaminated soil. The best formation effect of immobilized materials (n-HAP/SA) was found when the SA to n-HAP ratio was 3:2. When compared to single SA immobilized bacteria, n-HAP/SA immobilized bacteria improved the surface roughness and porosity of the microspheres. After 70 days, LED light revealed that the immobilized bacteria's GFP green fluorescent protein expression was stable. At 70 days, the initial DBP concentration of 500 mg ∙

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Author

Lei Wang; Xiaochen Jia; Zeyu Dou; Xiaoqian Li; Wenjing Bao; Chaoran Ma; Hongye Wang; Lei Wang; Maofeng Dong; Ying Zhang

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Environmental Science (Physical Sciences)